Come to terms with the fact that people will do whatever they want on Twitter, and there’s nothing you can do about it.
If you’re just linking to your blog and promoting yourself when you tweet, you risk alienating your valuable followers.
If you want people to pay attention to your tweets, make sure you actually have them in the first place.
Try to maintain a reasonable balance between how many people you follow and how many people follow you.
It isn’t necessary to update your Twitter account constantly; a little bit of space between tweets never hurt anybody.
Everyone can see what you post, so think twice before bashing your coworkers or spicing up your tweets with profanities.
Twitter’s @replies are certainly handy, but a long string of them can get confusing for everyone involved in the conversation or otherwise.